凌飞:南理工大学生物科学与工程学院副研究员-中文百科频道

人物经历

教育经历

1991-1995年，中山大学生命科学学院，动物学专业，学士学位

1999-2000年，华南师范大学生命科学院，进修生物化学与分子生物学研究生课程

2004、2008年，华南农业大学动物分子遗传专业，硕士、博士学位。

2008-2010年，华南理工大学生命科学与工程学院，博士后研究。

2013-2014年，美国华盛顿大学医学院基因组科学系，josh Akey课题组，进化基因组研究。

工作经历

1995年8月-1996年1月，海南椰风集团

2004年7月-2005年8月，华南农业大学动物科学学院陈瑶生教授课题组

2011年3月至今，华南理工大学生物学院

主要成就

项目成就

主持国家自然科学基金面上项目一项，作为子课题任务完成人主持国家科技支撑计划子课题一项，同时还主持省教育厅省级重大项目一项、中国博士后基金、广东省自然科学基金、广东省科技计划项目、中央高校基本科研业务费面上项目、重点项目和滚动项目等课题。参加国家基金重点项目一项。

近5年已发表SCI论文30余篇（第一作者或通讯作者身份发表SCI文章20篇，总影响因子91，单篇引用120次）。第一或通讯作者论文在Nature Biotechnology、PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES（Top期刊）、Scientific reports（二区）、virology、PLoS One、Tissue antigen、American journal of Primatology、Immunogenetics、human immunology、Molecular Biology Report、DNA and Cell Biology、Molecular and cell biochemistry、Biochemical and Biophysical Research Communications等杂志发表。

一篇开拓性文章《食蟹猴与中国恒河猴两种非人灵长类动物模型的基因组测序与比较》于2011年在《Nature Biotechnology》（影响因子43）在线发表。该重大成果的取得实现了华南理工大学生物学院教师问鼎自然系列杂志零的突破。在此基础上，本人在美国华盛顿大学留学期间，在领域内顶尖团队Josh Akey团队，通过灵长类动物基因组MHC区域DHSs比较、进化分析及结合GWAS数据库发现，除了宿主与病原体互作以及繁殖适应外，大脑发育与认知能力也在驱动人类MHC的进化，并从因果关系挖掘和证实了MHC的已有和潜在全新的功能。

回国后，在国内率先开展灵长类动物的DHS研究，聚焦宿主与病毒进化军备竞赛，首次从分子进化学角度阐述了灵长类动物TRIM家族76个潜在的抗病毒基因的调控区变异（DNA酶Ⅰ超敏感位点）如何应对长期进化过程中病毒的选择，发现并提出了灵长类动物基因组调控区变异与其抗病毒能力差异的进化机制。研究成果于2016年发表于SCI二区期刊Proceedings of the Royal Society B（《英国皇家学会会志B辑-生物科学》）上。

另外，通过对我国两个褐家鼠亚种精子甲基化组与基因组的深度测序及对比分析，证明了稳定环境差异（种间环境差异）导致的基因组甲基化差异可以在世代间传承，阐明了拉马克获得性与达尔文自然选择理论之间的关系，对物种分化的机制提出了全新的解释。

1、uangmei Yan§, Guojie Zhang§, Xiaodong Fang§, Yanfeng Zhang§, Cai Li§, Fei Ling§, David N Cooper, Qiye Li, Yan Li, Alain J van Gool, Hongli Du, Jiesi Chen, Ronghua Chen, Pei Zhang, Zhiyong Huang, John R Thompson, Yuhuan Meng, Yinqi Bai, Jufang Wang, Min Zhuo, Tao Wang, Ying Huang, Liqiong Wei, Jianwen Li, Zhiwen Wang, Haofu Hu, Pengcheng Yang, Liang Le, Peter D Stenson, Bo Li, Xiaoming Liu, Edward V Ball, Na An, Quanfei Huang, Yong Zhang, Wei Fan, Xiuqing Zhang, Yingrui Li, Wen Wang, Michael G Katze, Bing Su, Rasmus Nielsen, Huanming Yang, Jun Wang, Xiaoning Wang* & Jian Wang*. Genome sequencing and comparison of two non-human primate animal models, cynomolgus monkey and the Chinese rhesus macaque. Nature Biotechnology, 2011, 29(11): 1019-1023 (co-first author)（§同等贡献）(if:43)

2、He DD, Lu Y, Gittelman R, Jin Y, Ling F, Joshua A. Positive selection of the TRIM family regulatory region in primate genomes. Proc Biol Sci. 2016 Oct 12;283(1840). pii: 20161602(通讯作者）二区，Top期刊(if:5.336)

3、Su Z, Zhang J, Kumar C, Molony C, Lu H, Chen R, Stone DJ, Ling F, Liu X. Species-specific exome probes reveal new insights in positively selected genes in nonhuman primates. Sci Rep. 2016 Sep 23;6:33876. doi: 10.1038/srep33876.(共同通讯作者）二区(if:5.228)

4、Hui-Ling Zhang , Feng-Liang Liu , Ya-Bin Jin, Qing Deng, Bei-Lei Liu, Min Zhuo, Xiao-Hui liu, Yong-Tang Zheng, Fei Ling. The effects of TRIM5α polymorphism on HIV-2ROD and SIVmac239 replication in PBMCs from Chinese rhesus macaques and Vietnamese-origin cynomolgus macaques. Virology, Volume 487, January 2016, Pages 222–229(通讯作者）(if:3.2)

5、W. Wang, D.-D. He, S.-L. Mao, M. Zhuo, F. Ling. Ten novel MHC class II alleles identified in cynomolgus macaques of Vietnamese origin. Tissue Antigens HLA. 2016 Feb;87(2):115-6. doi: 10.1111/tan.12731. Epub 2016 Jan 13(通讯作者）

6、Chen M, Song P, Zou D, Hu X, Zhao S, Gao S, Ling F. Comparison of multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC) in single-cell sequencing. PLoS One. 2014 Dec 8;9(12)(通讯作者)

7、Wang W, Lu YE, Zhuo M, Ling F. Identification of five novel MHC class II alleles in cynomolgus macaques of Vietnamese origin. HLA. 2016 Jul;88(1-2):61-2. doi: 10.1111/tan.12840. Epub 2016 Jul 8(通讯作者)

8、Liu B, Jin Y, Lu Y, Wu Y, Xue Y, Zhang Y, Liu Y, Zhuo M, Ling F. Comprehensive identification of high-frequency and combination MHC-DMA and -DMB alleles in a cohort of Chinese rhesus macaques and cynomolgus macaques of Vietnamese origin. Hum Immunol. 2015 Mar;76(2-3):109-12.(通讯作者)

9、Zhang H, Deng Q, Jin Y, Liu B, Zhuo M, Ling F. Comprehensive identification of MHC class II alleles in a cohort of Chinese rhesus macaques. Immunogenetics. 2014 Oct; 66(9-10):575-80. doi: 10.1007/s00251-014-0789-y. Epub 2014 Jul 22(通讯作者)

10、Xiang R, Zhang H, Deng Q, Yue R, Tang H, Zhang Y, Ling F, Zhuo M, Du H, Xu S, Xu Q, Wang X. Comprehensive identification of high-frequency and co-occurring Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA alleles in Vietnamese cynomolgus macaques. Immunogenetics. 2013 Sep;65(9):667-74.(通讯作者）

11、Zhang G, Qiu W, Xiang R, Ling F, Zhuo M, Du H, Wang J, Wang X. TRIM5a Polymorphism Identification in Cynomolgus Macaques of Vietnamese Origin and Chinese Rhesus Macaques. Am J Primatol. 2013 Sep;75(9):938-46.(通讯作者）

12、Qing Deng , Huiling Zhang , Ruirui Xiang, Zhenwu Zhang , Fei Ling, Min Zhuo & Hongli Du & Xiaoning Wang. Identification of Mamu-DPA1, -DQA1, and -DRA alleles in a cohort of Chinese rhesus macaques. Immunogenetics. 2013 65:901–904(通讯作者）

13、Fei Ling, Min Zhuo, Chao Ni, Gui-qing Zhang, Tao Wang, Li-qiong Wei, Hong-li Du, Ju-fang Wang, Xiao-ning Wang. Comprehensive identification of high-frequency and co-occurring Mafa-B, Mafa-DQB1 and Mafa-DRB alleles in cynomolgus macaques of Vietnamese origin. Hum Immunol. 2012 May;73(5):547-53. doi: 10.1016/j.

14、Ling F, Wei LQ, Wang T, Wang HB, Zhuo M, Du HL, Wang JF, Wang XN. Characterization of the major histocompatibility complex class II DOB, DPB1, and DQB1 alleles in cynomolgus macaques of Vietnamese origin. Immunogenetics. (2011) 63:155–166.

15、Ling F, Wang T, Wei L, Zhu X, Chen Y, Li J, Zhang Z, Du H, Wang X, Wang J. Cloning and characterization of the 5'-flanking region of the pig AgRP gene. Mol Biol Rep. 2011 Apr;38(4):2233-9.

16、Ling F, Wei L, Wang T, Chen Y, Zhu X, Li J, Liu T, Du H, Wang H, Wang J. Cloning and Characterization of the 5'-Flanking Region of the Pig Cocaine- and Amphetamine-Regulated Transcript Gene. DNA Cell Biol. 2011 Feb;30(2):91-7.

17、H.-B. Wang§, F. Ling§, M. Zhuo, J. –F Wang, X. -N Wang. Twenty-three novel MHC class I B alleles identified in cynomolgus macaques of Vietnamese origin. Tissue Antigen, 2011,77, 341–348 (co- first author)（§同等贡献）

18、Ling F, Fang W, Chen Y, Li J, Liu X, Wang L, Zhang H, Chen S, Mei Y, Du H, Wang C. Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters . Mol Cell Biochem. 2010 Oct;343(1-2):239-47

19、Fei Ling, Jiaqi Li, Yaosheng Chen, Hongli Du, Yingjie Mei, Delin Mo, Chong Wang. Cloning and characterization of the 5′-flanking region of the pig adiponectin gene. Biochemical and Biophysical Research Communications, 2009 Apr 3;381(2):236-40.

20、Li W, Wang T, Ling F, Zhao H, Wei L, Zhuo M, Du H, Wang X. Identification of MhcMafa-DRB alleles in cohort of cynomolgus macaques of Vietnamese origin. Am J Primatol. 2012 Oct;74(10):958-66.

21、Zhuo M, Wang HB, Ling F, Wang JF, Wang XN. Eighteen novel MHC class I A alleles identified in Vietnamese-origin cynomolgus macaques. Tissue antigen 2011 Jun 6

22、Zhang GQ, Ni Ch, Ling F, Qiu W, Wang HB, Xiao Y, Guo XJ, Huang JY, Du HL, Wang JF, Zhao Sh J, Zhuo M, Wang XN. Characterization of the major histocompatibility complex class I A alleles in cynomolgus macaques of Vietnamese origin. Tissue Antigens. 2012 Dec;80(6):494-501.

23、Du H, Hu H, Meng Y, Zheng W, Ling F, Wang J, Zhang X, Nie Q, Wang X. The correlation coefficient of GC content of the genome-wide genes is positively correlated with animal evolutionary relationships. FEBS Lett. 2010, 584(18):3990-4

24、Zhang Z, Mo D, Ling F, Wang C, Li A, Zhao X, Li Y, Chen Y. Characterization and Promoter Activity Analysis of a New Porcine Gene: NICE-3. DNA Cell Biol. 2009 Nov 7.

25、Zhang Z, Mo D , Cong P, He Z, Ling F, Li A, Niu Y, Zhao X, Zhou C, Chen Y. Molecular cloning, expression patterns and subcellular localization of porcine TMCO1 gene. Mol Biol Rep. 2009 May 16. (if: 1.75)

26、Yingjie Mei; Yaosheng Chen; Jiaqi Li; Ping Gao; Chong Wang; Hao Zhang; Fei Ling; Yanfang Li; Shuihua Xie; Shixin Li; Gongqiu Zhang (2008), Sequence Identification, Tissue Distribution and Polymorphism of the PorcineCathepsin D (CTSD) Gene, Animal Biotechnology, 19:3,144 -158.

27、Li Y, Chen Y, Li J, Wang C, Liu X, Zhang H, Mei Y, Ling F, Li S, Chen S, Zhong W (2008), Molecular characterization, expression profile and polymorphisms of the porcine TNNC2 gene. Hereditas. Dec; 145(6):274-82. .

28、Ling F, Li JQ, Wang C, Liu C, DU HL, Xiao ZZ, Wang LL, Chen YS. Methylation status of pig adiponectin promoter and its expression. Yi Chuan. 2009 Oct;31(10):1013-9.

29、Chen J, Meng Y, Zhou J, Zhuo M, Ling F, Zhang Y, Du H, Wang X.Identifying candidate genes for Type 2 Diabetes Mellitus and obesity through gene expression profiling inmultiple tissues or cells. J Diabetes Res. 2013;2013:970435. doi: 10.1155/2013/970435. Epub 2013 Dec 26.

30、Jin X, He M, Ferguson B, Meng Y, Ouyang L, Ren J, Mailund T, Sun F, Sun L, Shen J, Zhuo M, Song L, Wang J, Ling F, Zhu Y, Hvilsom C, Siegismund H, Liu X, Gong Z, Ji F, Wang X, Liu B, Zhang Y, Hou J, Wang J, Zhao H, Wang Y, Fang X, Zhang G, Wang J, Zhang X, Schierup MH, Du H, Wang J, Wang X. An effort to use human-based exome capture methods to analyze chimpanzee and macaque exomes.PLoS One. 2012;7(7):e40637. doi: 10.1371/journal.pone.0040637. Epub 2012 Jul 27