淩飛:南理工大學生物科學與工程學院副研究員-中文百科頻道

人物經曆

教育經曆

1991-1995年，中山大學生命科學學院，動物學專業，學士學位

1999-2000年，華南師範大學生命科學院，進修生物化學與分子生物學研究生課程

2004、2008年，華南農業大學動物分子遺傳專業，碩士、博士學位。

2008-2010年，華南理工大學生命科學與工程學院，博士後研究。

2013-2014年，美國華盛頓大學醫學院基因組科學系，josh Akey課題組，進化基因組研究。

工作經曆

1995年8月-1996年1月，海南椰風集團

2004年7月-2005年8月，華南農業大學動物科學學院陳瑤生教授課題組

2011年3月至今，華南理工大學生物學院

主要成就

項目成就

主持國家自然科學基金面上項目一項，作為子課題任務完成人主持國家科技支撐計劃子課題一項，同時還主持省教育廳省級重大項目一項、中國博士後基金、廣東省自然科學基金、廣東省科技計劃項目、中央高校基本科研業務費面上項目、重點項目和滾動項目等課題。參加國家基金重點項目一項。

近5年已發表SCI論文30餘篇（第一作者或通訊作者身份發表SCI文章20篇，總影響因子91，單篇引用120次）。第一或通訊作者論文在Nature Biotechnology、PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES（Top期刊）、Scientific reports（二區）、virology、PLoS One、Tissue antigen、American journal of Primatology、Immunogenetics、human immunology、Molecular Biology Report、DNA and Cell Biology、Molecular and cell biochemistry、Biochemical and Biophysical Research Communications等雜志發表。

一篇開拓性文章《食蟹猴與中國恒河猴兩種非人靈長類動物模型的基因組測序與比較》于2011年在《Nature Biotechnology》（影響因子43）在線發表。該重大成果的取得實現了華南理工大學生物學院教師問鼎自然系列雜志零的突破。在此基礎上，本人在美國華盛頓大學留學期間，在領域内頂尖團隊Josh Akey團隊，通過靈長類動物基因組MHC區域DHSs比較、進化分析及結合GWAS數據庫發現，除了宿主與病原體互作以及繁殖适應外，大腦發育與認知能力也在驅動人類MHC的進化，并從因果關系挖掘和證實了MHC的已有和潛在全新的功能。

回國後，在國内率先開展靈長類動物的DHS研究，聚焦宿主與病毒進化軍備競賽，首次從分子進化學角度闡述了靈長類動物TRIM家族76個潛在的抗病毒基因的調控區變異（DNA酶Ⅰ超敏感位點）如何應對長期進化過程中病毒的選擇，發現并提出了靈長類動物基因組調控區變異與其抗病毒能力差異的進化機制。研究成果于2016年發表于SCI二區期刊Proceedings of the Royal Society B（《英國皇家學會會志B輯-生物科學》）上。

另外，通過對我國兩個褐家鼠亞種精子甲基化組與基因組的深度測序及對比分析，證明了穩定環境差異（種間環境差異）導緻的基因組甲基化差異可以在世代間傳承，闡明了拉馬克獲得性與達爾文自然選擇理論之間的關系，對物種分化的機制提出了全新的解釋。

1、uangmei Yan§, Guojie Zhang§, Xiaodong Fang§, Yanfeng Zhang§, Cai Li§, Fei Ling§, David N Cooper, Qiye Li, Yan Li, Alain J van Gool, Hongli Du, Jiesi Chen, Ronghua Chen, Pei Zhang, Zhiyong Huang, John R Thompson, Yuhuan Meng, Yinqi Bai, Jufang Wang, Min Zhuo, Tao Wang, Ying Huang, Liqiong Wei, Jianwen Li, Zhiwen Wang, Haofu Hu, Pengcheng Yang, Liang Le, Peter D Stenson, Bo Li, Xiaoming Liu, Edward V Ball, Na An, Quanfei Huang, Yong Zhang, Wei Fan, Xiuqing Zhang, Yingrui Li, Wen Wang, Michael G Katze, Bing Su, Rasmus Nielsen, Huanming Yang, Jun Wang, Xiaoning Wang* & Jian Wang*. Genome sequencing and comparison of two non-human primate animal models, cynomolgus monkey and the Chinese rhesus macaque. Nature Biotechnology, 2011, 29(11): 1019-1023 (co-first author)（§同等貢獻）(if:43)

2、He DD, Lu Y, Gittelman R, Jin Y, Ling F, Joshua A. Positive selection of the TRIM family regulatory region in primate genomes. Proc Biol Sci. 2016 Oct 12;283(1840). pii: 20161602(通訊作者）二區，Top期刊(if:5.336)

3、Su Z, Zhang J, Kumar C, Molony C, Lu H, Chen R, Stone DJ, Ling F, Liu X. Species-specific exome probes reveal new insights in positively selected genes in nonhuman primates. Sci Rep. 2016 Sep 23;6:33876. doi: 10.1038/srep33876.(共同通訊作者）二區(if:5.228)

4、Hui-Ling Zhang , Feng-Liang Liu , Ya-Bin Jin, Qing Deng, Bei-Lei Liu, Min Zhuo, Xiao-Hui liu, Yong-Tang Zheng, Fei Ling. The effects of TRIM5α polymorphism on HIV-2ROD and SIVmac239 replication in PBMCs from Chinese rhesus macaques and Vietnamese-origin cynomolgus macaques. Virology, Volume 487, January 2016, Pages 222–229(通訊作者）(if:3.2)

5、W. Wang, D.-D. He, S.-L. Mao, M. Zhuo, F. Ling. Ten novel MHC class II alleles identified in cynomolgus macaques of Vietnamese origin. Tissue Antigens HLA. 2016 Feb;87(2):115-6. doi: 10.1111/tan.12731. Epub 2016 Jan 13(通訊作者）

6、Chen M, Song P, Zou D, Hu X, Zhao S, Gao S, Ling F. Comparison of multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC) in single-cell sequencing. PLoS One. 2014 Dec 8;9(12)(通訊作者)

7、Wang W, Lu YE, Zhuo M, Ling F. Identification of five novel MHC class II alleles in cynomolgus macaques of Vietnamese origin. HLA. 2016 Jul;88(1-2):61-2. doi: 10.1111/tan.12840. Epub 2016 Jul 8(通訊作者)

8、Liu B, Jin Y, Lu Y, Wu Y, Xue Y, Zhang Y, Liu Y, Zhuo M, Ling F. Comprehensive identification of high-frequency and combination MHC-DMA and -DMB alleles in a cohort of Chinese rhesus macaques and cynomolgus macaques of Vietnamese origin. Hum Immunol. 2015 Mar;76(2-3):109-12.(通訊作者)

9、Zhang H, Deng Q, Jin Y, Liu B, Zhuo M, Ling F. Comprehensive identification of MHC class II alleles in a cohort of Chinese rhesus macaques. Immunogenetics. 2014 Oct; 66(9-10):575-80. doi: 10.1007/s00251-014-0789-y. Epub 2014 Jul 22(通訊作者)

10、Xiang R, Zhang H, Deng Q, Yue R, Tang H, Zhang Y, Ling F, Zhuo M, Du H, Xu S, Xu Q, Wang X. Comprehensive identification of high-frequency and co-occurring Mafa-DPA1, Mafa-DQA1, Mafa-DRA, and Mafa-DOA alleles in Vietnamese cynomolgus macaques. Immunogenetics. 2013 Sep;65(9):667-74.(通訊作者）

11、Zhang G, Qiu W, Xiang R, Ling F, Zhuo M, Du H, Wang J, Wang X. TRIM5a Polymorphism Identification in Cynomolgus Macaques of Vietnamese Origin and Chinese Rhesus Macaques. Am J Primatol. 2013 Sep;75(9):938-46.(通訊作者）

12、Qing Deng , Huiling Zhang , Ruirui Xiang, Zhenwu Zhang , Fei Ling, Min Zhuo & Hongli Du & Xiaoning Wang. Identification of Mamu-DPA1, -DQA1, and -DRA alleles in a cohort of Chinese rhesus macaques. Immunogenetics. 2013 65:901–904(通訊作者）

13、Fei Ling, Min Zhuo, Chao Ni, Gui-qing Zhang, Tao Wang, Li-qiong Wei, Hong-li Du, Ju-fang Wang, Xiao-ning Wang. Comprehensive identification of high-frequency and co-occurring Mafa-B, Mafa-DQB1 and Mafa-DRB alleles in cynomolgus macaques of Vietnamese origin. Hum Immunol. 2012 May;73(5):547-53. doi: 10.1016/j.

14、Ling F, Wei LQ, Wang T, Wang HB, Zhuo M, Du HL, Wang JF, Wang XN. Characterization of the major histocompatibility complex class II DOB, DPB1, and DQB1 alleles in cynomolgus macaques of Vietnamese origin. Immunogenetics. (2011) 63:155–166.

15、Ling F, Wang T, Wei L, Zhu X, Chen Y, Li J, Zhang Z, Du H, Wang X, Wang J. Cloning and characterization of the 5'-flanking region of the pig AgRP gene. Mol Biol Rep. 2011 Apr;38(4):2233-9.

16、Ling F, Wei L, Wang T, Chen Y, Zhu X, Li J, Liu T, Du H, Wang H, Wang J. Cloning and Characterization of the 5'-Flanking Region of the Pig Cocaine- and Amphetamine-Regulated Transcript Gene. DNA Cell Biol. 2011 Feb;30(2):91-7.

17、H.-B. Wang§, F. Ling§, M. Zhuo, J. –F Wang, X. -N Wang. Twenty-three novel MHC class I B alleles identified in cynomolgus macaques of Vietnamese origin. Tissue Antigen, 2011,77, 341–348 (co- first author)（§同等貢獻）

18、Ling F, Fang W, Chen Y, Li J, Liu X, Wang L, Zhang H, Chen S, Mei Y, Du H, Wang C. Identification of novel transcripts from the porcine MYL1 gene and initial characterization of its promoters . Mol Cell Biochem. 2010 Oct;343(1-2):239-47

19、Fei Ling, Jiaqi Li, Yaosheng Chen, Hongli Du, Yingjie Mei, Delin Mo, Chong Wang. Cloning and characterization of the 5′-flanking region of the pig adiponectin gene. Biochemical and Biophysical Research Communications, 2009 Apr 3;381(2):236-40.

20、Li W, Wang T, Ling F, Zhao H, Wei L, Zhuo M, Du H, Wang X. Identification of MhcMafa-DRB alleles in cohort of cynomolgus macaques of Vietnamese origin. Am J Primatol. 2012 Oct;74(10):958-66.

21、Zhuo M, Wang HB, Ling F, Wang JF, Wang XN. Eighteen novel MHC class I A alleles identified in Vietnamese-origin cynomolgus macaques. Tissue antigen 2011 Jun 6

22、Zhang GQ, Ni Ch, Ling F, Qiu W, Wang HB, Xiao Y, Guo XJ, Huang JY, Du HL, Wang JF, Zhao Sh J, Zhuo M, Wang XN. Characterization of the major histocompatibility complex class I A alleles in cynomolgus macaques of Vietnamese origin. Tissue Antigens. 2012 Dec;80(6):494-501.

23、Du H, Hu H, Meng Y, Zheng W, Ling F, Wang J, Zhang X, Nie Q, Wang X. The correlation coefficient of GC content of the genome-wide genes is positively correlated with animal evolutionary relationships. FEBS Lett. 2010, 584(18):3990-4

24、Zhang Z, Mo D, Ling F, Wang C, Li A, Zhao X, Li Y, Chen Y. Characterization and Promoter Activity Analysis of a New Porcine Gene: NICE-3. DNA Cell Biol. 2009 Nov 7.

25、Zhang Z, Mo D , Cong P, He Z, Ling F, Li A, Niu Y, Zhao X, Zhou C, Chen Y. Molecular cloning, expression patterns and subcellular localization of porcine TMCO1 gene. Mol Biol Rep. 2009 May 16. (if: 1.75)

26、Yingjie Mei; Yaosheng Chen; Jiaqi Li; Ping Gao; Chong Wang; Hao Zhang; Fei Ling; Yanfang Li; Shuihua Xie; Shixin Li; Gongqiu Zhang (2008), Sequence Identification, Tissue Distribution and Polymorphism of the PorcineCathepsin D (CTSD) Gene, Animal Biotechnology, 19:3,144 -158.

27、Li Y, Chen Y, Li J, Wang C, Liu X, Zhang H, Mei Y, Ling F, Li S, Chen S, Zhong W (2008), Molecular characterization, expression profile and polymorphisms of the porcine TNNC2 gene. Hereditas. Dec; 145(6):274-82. .

28、Ling F, Li JQ, Wang C, Liu C, DU HL, Xiao ZZ, Wang LL, Chen YS. Methylation status of pig adiponectin promoter and its expression. Yi Chuan. 2009 Oct;31(10):1013-9.

29、Chen J, Meng Y, Zhou J, Zhuo M, Ling F, Zhang Y, Du H, Wang X.Identifying candidate genes for Type 2 Diabetes Mellitus and obesity through gene expression profiling inmultiple tissues or cells. J Diabetes Res. 2013;2013:970435. doi: 10.1155/2013/970435. Epub 2013 Dec 26.

30、Jin X, He M, Ferguson B, Meng Y, Ouyang L, Ren J, Mailund T, Sun F, Sun L, Shen J, Zhuo M, Song L, Wang J, Ling F, Zhu Y, Hvilsom C, Siegismund H, Liu X, Gong Z, Ji F, Wang X, Liu B, Zhang Y, Hou J, Wang J, Zhao H, Wang Y, Fang X, Zhang G, Wang J, Zhang X, Schierup MH, Du H, Wang J, Wang X. An effort to use human-based exome capture methods to analyze chimpanzee and macaque exomes.PLoS One. 2012;7(7):e40637. doi: 10.1371/journal.pone.0040637. Epub 2012 Jul 27